Brief Communication: The FIND-IT technology
🚨 Exciting Research Alert! 🚨
New publication out: Just FIND-IT: Harnessing the true power of induced mutagenesis.
In collaboration with key researchers at the Carlsberg Group Research Laboratory, we have a Brief Communication in Plant Biotechnology Journal.
🌟 You can find it here: https://shorturl.at/ORBCM 🌟
The FIND-IT technology was first published in Science Advances in 2022 (Knudsen et al., 2022: https://lnkd.in/dNZrpjU6) and provides an agile and high-throughput approach to screen unprecedented large size chemically-induced variant populations at single-nucleotide resolution
As recently published, this non-GMO approach has enabled us to disentangle hydroxynitrile glucoside biosynthesis in barley and thereby to provide access to elite malting barleys for ethyl carbamate-free whisky production (Jørgensen et al, 2024: https://lnkd.in/dEPu9iyt).
The FIND-IT technology has also been instrumental in obtaining sweet seeds in white lupin devoid of anti-nutritional alkaloids of the quinolizidine type, in eliminating the presence of anti-nutritional saponins in quinoa seeds, in improving phosphate bioavailability in the barley grain and in modifying the flavor profiles of yeast species for use in industrial brewing.
In total, more than 500,000 FIND-IT barley variant plants are today available for screening. This means that virtually all putative mutation sites in the plant variant population have been saturated. When the sites are saturated, their status shifts from being random to become available as defined distinct sites in one or more individual plants present in the variant collection. Accordingly, the individual grains of the plant carrying a specific desired SNP in a specific gene may be identified from the grain pools using a TaqManTM assay and digital PCR. You know the mutation is there and just have to FIND-IT!!!!.
In the Brief Communication we summarize the procedure based on knowledge of the mutant load and spectrum. In this way, we hope to engage many more researchers to use the FIND-IT technology to obtain new desired traits to ensure the development of resilient and environmentally benign production systems for the future.
A big thank you to my collaborators!!
Christoph Dockter
Søren Knudsen
Magnus Rasmussen
Birgitte Skadhauge
Carlsberg Foundation