Katrine Rønne Rostgaard
Vertical arrays of inorganic nanowires (NWs) are arrays of solid, inorganic NWs vertically aligned on a solid surface. The NWs are typically 60-100 nm in diameter and 2-10 µm long. When interfaced with living mammalian cells the NWs interact closely with the cells, and many different cell types have been shown to be viable for several days after seeding on the NWs.
My PhD project aims to use NWs to deliver biosensing molecules into cells. This is interesting because many methods of cellular investigations require delivery of large biosensing molecules to report on the activation of cellular pathways. These compounds can traditionally only be delivered into cells by permeabilizing the cell membrane, i.e. often at the cost of the life of the cell. Using NWs to deliver these molecules into cells would thus allow studies to be performed on living cells.
I study the cell-NW interaction by fluorescence microscopy and also work with functionalizing the NW surface to improve delivery into cells and with immobilization of proteins on the NWs.
|2011- ongoing||PhD student in Bionanoscience|
|2009-2011||Master in Nanoscience. (Master thesis title: Bimolecular Fluorescence Complementation (BiFC) based nanowire internalization assay)|
|2005-2009||Bachelor in Nanotechnology|
Selected Scientific Publications
Frederiksen, R., Alarcon-Llado, E., Madsen, M.H., Rostgaard, K.R., Krogstrup, P., Vosch, T., Nygård, J., Fontcuberta i Morral, A. & Martinez, K.L. Modulation of fluorescence signals from biomolecules along nanowires due to interaction of light with oriented nanostructures Nanoletters 15 (1), 176- 181doi: 10.1021/nl503344y (2015)
Frederiksen, R., Alarcon-Llado, E., Madsen, M.H., Rostgaard, K.R., Krogstrup, P., Vosch, T., Nygård, J., Fontcuberta, I., Morral, A., Martinez, K.L.
Modulation of fluorescence signals from biomolecules along nanowires due to interaction of light with oriented nanostructures. Nano Letters, doi:10.1021/nl503344y (2014). Epub ahead of print
Bonde, S., Buch-Månson, N., Rostgaard, K.R., Andersen, T.K., Berthing, T., Martinez, K.L. Exploring arrays of vertical one-dimensional nanostructures for cellular investigations. Nanotechnology 25(36):362001, doi:10.1088/0957-4484/25/36/362001 (2014).
Rostgaard, K.R., Frederiksen, R.S., Liu, Y.C., Berthing, T., Madsen, M.H., Holm, J., Nygård, J., Martinez, K.L. Vertical nanowire arrays as a versatile platform for protein detection and analysis. Nanoscale 5(21):10226-35, doi:10.1039/c3nr03113f (2013).
Berthing, T., Bonde, S., Rostgaard, K.R., Madsen, M.H., Sørensen, C.B., Nygård, J., Martinez, K.L. Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging. Nanotechnology 23(41):415102, doi:10.1088/0957-4484/23/41/415102 (2012).